Firstly, the Amide I band, which is in the region of 1,600-1,700 cm -1, is primarily due to the amide C=O stretching vibrations of the peptide bonds. The protein backbone amide groups generate a number of characteristic IR bands that can be used to determine protein backbone conformation and secondary structure. For proteins, the FTIR spectrum is composed of many vibrational bands arising from different functional groups such as N-H, C=O, and so on. Creative Biolabs offers Fourier transform infrared (FTIR) spectroscopy for analysis of antibody secondary structure.įTIR spectroscopy is an absorption spectroscopy that can be used to obtain information about the vibrational states of molecules. Particularly, high-order structure (HOS) is one of the critical quality attributes (CQAs) that should be characterized according to the ICH Q6B guideline. A variety of analytical tools have been used to give a comprehensive characterization of mAb candidates in terms of their biophysical properties, stability, affinity, etc, to ensure product efficacy and safety. Monoclonal antibodies (mAbs) are the most common protein that is being developed by many companies as therapies against a wide range of diseases. Pharmacology and Pharmacodynamics Studies.Developability Assessment of Biopharmaceutical Candidates.Small-Angle X-ray and Neutron Scattering. Nuclear Magnetic Resonance (NMR) Spectroscopy.Natural Bioactive Compound Target Identification.In Silico Functional Analysis for Fungi.
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